The cornerstone of successful experiments is sample preparation. The products we distribute and develop are thoroughly tested and evaluated to ensure simple, efficient and reproducible sample preparation procedure.

MagResyn Protein A

High capacity Protein A for affinity purification of antibodies and immunoprecipitation

MagReSyn® Protein A microspheres contain covalently coupled Protein A for highly specific single-step purification of IgG to >95% purity from whole serum samples. Protein A is a ~56 kDa surface protein originally isolated from the cell wall of the bacterium Staphylococcus aureus and is widely used for highly specific purification of IgG antibodies and for immunoprecipitation experiments. Protein A has 5 immunoglobulin binding domains folded into a three-helix bundle and specifically binds the Fc region on the heavy chain of the IgG class of antibodies. More specifically, Protein A binds with high affinity to specific subclasses of human, rabbit, mouse, guinea pig, cat and pig IgG. It does not react with human IgG3, IgD or IgE, mouse IgM, IgA or IgE, or goat, koala and llama IgG. The high recombinant Protein A content of MagReSyn® ensures a high immunoglobulin capacity allowing for experimental miniaturization.

 

Support : Proprietary polymer microspheres containing iron oxide (magnetite) with immobilized Protein A

Binding capacity : > 2.4 mg.ml-1 (Rabbit IgG)

Bead size : 5-10 µm average

Formulation : 15 mg.ml-1 suspension in Tris buffer

2 mL : 370 $

5 mL : 735 $

10 mL : 1175 $

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